Isolation of Burkholderia cepacia complex bacteria from environmental and clinical samples and evaluation of their antibiotic susceptibility

Author: Nino Kalichava
Keywords: Burkholderia cepacia complex, nosocomial diseases, antibiotic susceptibility
Annotation:

The Burkholderia cepacia complex includes up to 30 closely related opportunistic pathogenic species. They are Gram-negative rods that naturally inhabit water and soil environments and are characterized by natural resistance to antibiotics. It should be noted that representatives of this complex play an important role in the development of nosocomial diseases. With the increase of resistance to antibiotics, nosocomial infections caused by Bcc complex are a serious challenge for the world health field in the 21st century. The aim of our study was to isolate bacteria belonging to the Burkholderia cepacia complex from clinical (hospital environment and patients with respiratory tract bacterial infections) and environmental (water and soil) samples and to study their sensitivity to antibiotics. In my bachelor's thesis, I discuss in detail the methods of isolation of the target bacterial strains, the process of their phenotypic identification and the sensitivity of the identified bacterial strains to 17 selected antibiotics.