Calorimetric and spectrophotometric study of calcium-containing DPPC and DPPA liposomes

Author: Salome Chinchaladze
Keywords: Liposomes, Nanoparticles, Calorimetry, Spectrophotometry
Annotation:

In the study, both uncomplexed and calcium-containing complex liposomes made of DPPA and DPPC phospholipids were studied calorimetrically and spectrophotometrically. Liposomes with a diameter of 200 nm were produced by new nanotechnology. Complex liposomes were prepared in both water and 20% glycerol solvent. As calorimetric and spectrophotometric experiments show, CaCl2 molecules made by new technology are incorporated into the structure of liposomes, which allows them to be used for treatment. In addition, calcium is placed in large quantities in such complex liposomes, although their entry into the blood does not cause side effects. The structure and thermal stability of complex liposomes were determined in the paper. In particular, according to the obtained experimental results, we believe that the structure of cal-cium-containing nanoparticles can be both unilaminar and multilaminar, in particular, the structure of uncomplexed and calcium-containing DPPC liposomes prepared in water and glycerol are mul-tilaminar. The structure of uncomplexed DPPA liposomes prepared in water is unilaminar, while calcium-containing DPPA liposomes prepared in water form a multilayer structure. As for the structure of both pure and calcium-containing DPPA liposomes prepared in glycerol are multilamellar.