Development of an enantioselective bioanalytical method for N-ethylhexedrone and its metabolites using liquid chromatography-mass spectrometry

As the number of illegal drugs and various pharmaceutical drugs increases at a rapid pace, the role of enantiocell analysis in pharmaceutical chemistry, toxicology, and other fields is becoming increasingly important. Enantioselective high-efficiency liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) more or less adequately Satisfies the requirements in these areas and has the potential to assist the researcher in identifying foreign substances entering the human body and correct interpretation of substance abuse. As our experience shows, there are certain technical issues that scientists need to consider in order to properly conduct an enantiocell HPLC-MS/MS experiment. This thesis is a kind of summary of our observations in this regard. The purpose of the thesis is to process the enantioselective bioanalytical method of N-ethylhexedron and its metabolites in human blood, oral fluid and urine samples using liquid chromatography-tandem mass spectrometry. In the beginning we did screening with several stationary and mobile phases. In the experiment, we used methanol-water 60-40% as a mobile phase with 5 millimolar ammonium acetate and Lux Cellulose-1 as a chiral column.