Study of migration order of brompherinamine enantiomers in capillary electrophoresis using charged cyclodextrins as chiral selectors

Nowadays, more than half of the molecules of herbal substances contain a chiral center, most of them are used as racemates, which consist of equimolecular amounts of enantiomers. By the way Enantiomers, on the other hand, have the same chemical composition and physico-chemical properties, but often exhibit different metabolic, pharmacological, toxicological, etc. actions. It is also worth noting the fact that the human body contains a large amount of homochilar compounds, which is why it is a powerful chiral selector and reacts differently to different enantiomers. Thus, one isomer may exhibit positive pharmacological and therapeutic effects while the other enantiomer may exhibit severe toxic effects. Therefore, before the registration of the pharmaceutical product, it is necessary to study the activity of the enantiomer, which enantiomer will show a positive therapeutic effect, and only this enantiomer should be used in its pure as a medicinal product. Therefore, the study of the separation of the mixture of enantiomers is one of the topical topics today. Capillary electrophoresis is one of the powerful methods of enantiomer separation. Its advantage lies in the following: it is characterized by high efficiency, the analysis process requires little time, belongs to miniature methods that do not pollute the environment. Also, the capillary is voltage resistant, which allows high voltages to be used at the expense of generating a small amount of heat. The use of such a voltage ensures high efficiency of the method, short analysis time and high resolution. In capillary electrophoresis, separation is done using chiral selectors. In this method, cyclodextrins are used as chiral selectors, which are characterized by universal capabilities of chiral detection. Cyclodextrins dissolve well in aqueous buffers and are not toxic substances. Their interaction with chiral molecules may take place by a different stereoselective mechanism. Cyclodextrins enantioselectively resolve enantiomers by forming non-covalent diastereomeric complexes. The resulting complexes differ from each other in spatial orientation or specific intermolecular interaction forces, through which the difference between the electrophoretic shifts and the separation of enantiomers is made

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