Study of the Impact on Natural Polyphenolic Compounds on liver Pathology Induced by a High-fat Diet Combined with Streptozotocin

Author: Mariam Gogiberidze
Keywords: .Streptozotocin, High-fat diet, ALT/AST, Cholesterol, Triglycerides
Annotation:

Metabolic syndrome-associated fatty liver disease represents a global health problem with increasing prevalence annually. According to statistical data, in the 1990s, the disease rate was 18.2% of the world's population, while for 2020-2024 it comprises 40%, and in approximately 10 years, its rate may exceed 50% of the world's population. Currently, there is no precise information about the full spectrum of metabolic disorders involved in the disease's pathogenesis. Moreover, there is no direct pharmacological treatment for the disease. Since this pathology is mainly associated with peroxidation, the search for its therapeutic and preventive means is directed towards studying antioxidant compounds, including polyphenols, in relation to the aforementioned pathology. Therefore, the aim of our study was to investigate the effects of polyphenolic compounds on organ-specific functional markers in model animal organisms during liver pathology induced by a high-fat diet and streptozotocin. The animal study was conducted in compliance with ethical standards and consisted of several stages: In the initial stage, we obtained a pathology model in laboratory rats using streptozotocin and a high-fat diet, confirming the establishment of pathology using appropriate markers. We then injected polyphenolic compounds into these experimental models. Specifically, a polyphenolic fraction extracted by us from grape seeds and chemically pure quercetin were administered. In the final stage, we studied the antioxidant properties of polyphenolic compounds and quercetin in plasma by determining the relevant biochemical markers (ALT, AST, cholesterol, triglycerides). The results confirmed that during liver pathology induced by a high-fat diet and streptozotocin in rats, the concentration of liver enzymes in plasma returns to the initial physiological level as a result of the effects of quercetin and polyphenolic fraction, unlike the changes in cholesterol and triglycerides. Considering the results, we can conclude that during liver pathology obtained by a high-fat diet and streptozotocin in rats, polyphenolic compounds show a positive effect on the liver ALT/AST functional indicator, while in the case of cholesterol and triglycerides, a 10-day exposure to polyphenols does not show an effect on their metabolism.

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Study of the Impact on Natural Polyphenolic Compounds on liver Pathology Induced by a High-fat Diet Combined with Streptozotocin [en]
მაღალცხიმოვანი დიეტითა და სტრეპტოზოტოცინით გამოწვეული ღვიძლის პათოლოგიის დროს ბუნებრივი ბიოაქტიური პოლიფენოლური ნაერთების ზეგავლენის შესწავლა ვირთაგვებში [ka]