A biophysical study of chitosan-coated liposomes

Author: Salome Chinchaladze
Keywords: Liposomes, Nanoparticles
Annotation:

Liposomes have been used as delivery vehicles for stabilizing drugs, overcoming barriers to cellular and tissue uptake, and for directing their contents toward specific sites in vivo. Chitosan is a biocompatible, biodegradable, and nontoxic cationic polysaccharide . Owing to its properties, chitosan can be used in medicine, pharmacy, biotechnology, agriculture, and the food industry.In the present work, the interaction between chitosan and dipalmitoyl phosphatidylcholine (DPPC) liposomes was studied by zeta potential, solubilization using the nonionic detergent octylglucoside (OG), as well as Fourier transform infrared (FTIR) spectroscopy. The coating of DPPC liposomes by a chitosan layer was confirmed by electron microscope images and the zeta potential of liposomes. Coating of liposome by chitosan resulted in an increase in liposomal size by addition of a layer of 92 ± 27.1 nm. The liposomal zeta potential became increasingly positive then it held at a relatively constant value.